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Fig. 3 | Gut Pathogens

Fig. 3

From: Bioengineering of LAB vector expressing Haemolysin co-regulated protein (Hcp): a strategic approach to control gut colonization of Campylobacter jejuni in a murine model

Fig. 3

Oral immunization schedule and analysis of local and cellular immune responses in experimental mice. Schematic of the mice immunization schedule. On day 7 post last immunization, of ten mice, three mice from each group were sacrificed to obtain spleen, whereas the remaining mice were orally challenged with 2 × 109 CFU of C. jejuni. All the challenged mice were sacrificed on day 35 to collect cecal tissue and its contents (a). Comparative assessment of Hcp specific local antibody (sIgA) level in fecal soups collected on day 7 post last immunization shows a substantial increment of sIgA level in the groups treated with CWA-Hcp and Sec-Hcp. Each bar represents the mean absorbance (A450) ± SE of 12 mice from three independent experiments (four mice from each experimental setup). Asterisks indicate a statistically significant difference (**P ≤ 0.01) compared to the control group (PBS only) (b). However, no such change was observed in the serum IgG level among the groups. ‘ns’ represents a non-significant difference between different experimental groups. (c) Splenocytes collected from different experimental groups were stimulated with 10 μg/mL of rHcp. For each experiment, a total of three animals were randomly selected from two independent experiments performed under similar conditions (n = 6). Each assay was performed in triplicates, and the absorbance was measured at 595 nm. The bar represents the mean stimulation index ± SE of six mice. Asterisks indicate a statistically significant difference in the treated group (**P ≤ 0.01) with respect to the control group (PBS only) (d). A pairwise statistical comparison among the groups was presented using a “P-value matrix” (b, c, d). The darker boxes represent the P-value closer to 0.01 (significant), while the lighter boxes represent P-value closer to 1 (non-significant)

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