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Fig. 4 | Gut Pathogens

Fig. 4

From: Bioengineering of LAB vector expressing Haemolysin co-regulated protein (Hcp): a strategic approach to control gut colonization of Campylobacter jejuni in a murine model

Fig. 4

Nitric oxide (NO) production by splenocyes. The nitrite concentration in the supernatant of splenocytses culture was determined against sodium nitrite (NaNO2) as a standard. For this first a standard curve of nitrite concentration (x-axis) against absorbance (y-axis) was plotted (a). At day 7 post last immunization, production of NO was determined by adding Griess reagent in the culture supernatant of splenocytes collected from different experimental mice and stimulated with varying concentrations of rHcp (0.1 μg/mL, 1 μg/mL, and 5 μg/mL). Concanavalin A (ConA) served as a positive control. For each experiment, a total of three mice were randomly selected from two independent experiments performed under similar conditions (n = 6). Data represent the mean NO production ± SE of six mice from two independent experiments. Asterisks indicate a statistically significant difference (*P ≤ 0.05, **P ≤ 0.01) compared to the control (received PBS only) (b)

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Gut Pathogens

ISSN: 1757-4749