Table 2 List of primers used in this study
| Target Gene | Primer Sequence (5′-3′) | Amplicon size (bp) | Ref |
|---|---|---|---|
| pQE30-hcp |
F-5′ CCGCGGTACCATGG CTGAACCAGCGTTTATAAAAATTG 3′ R-5′ GACTACTGCAGTT AAGCTTTGCCCTCTCTCCA 3′ | 510 | [31] |
| pNZ8048-hcp |
F-5′ CATCCGCGGATGGCTGAACCAGCGTTT 3′ R-5′ GATGCTAGCGAGCTTTGCCCTCTCTCCA 3′ | 529 | This work |
| pSEC-hcp |
F-5′ CGCATGCATATGGCTGAACCAGCGTTTATA 3′ R-5′ CAGGAGCTCTTA AAGCTTTGCCCTCTCTCC 3′ | 529 | This work |