Table 2 List of primers used in this study
Target Gene | Primer Sequence (5′-3′) | Amplicon size (bp) | Ref |
|---|---|---|---|
pQE30-hcp | F-5′ CCGCGGTACCATGG CTGAACCAGCGTTTATAAAAATTG 3′ R-5′ GACTACTGCAGTT AAGCTTTGCCCTCTCTCCA 3′ | 510 | [31] |
pNZ8048-hcp | F-5′ CATCCGCGGATGGCTGAACCAGCGTTT 3′ R-5′ GATGCTAGCGAGCTTTGCCCTCTCTCCA 3′ | 529 | This work |
pSEC-hcp | F-5′ CGCATGCATATGGCTGAACCAGCGTTTATA 3′ R-5′ CAGGAGCTCTTA AAGCTTTGCCCTCTCTCC 3′ | 529 | This work |