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Fig. 6 | Gut Pathogens

Fig. 6

From: Microbiome diversity declines while distinct expansions of Th17, iNKT, and dendritic cell subpopulations emerge after anastomosis surgery

Fig. 6

Increase in iNKT cells expressing IL-10 and IL-22 after end-to-end and Roux-en-Y anastomoses. Intestinal mucosal cells were harvested 3 days post-operatively and stained extracellularly with TCRβ and CD1dtet and intracellularly with IL-10, IL-22, IL-17A, and IL-17F. Samples were then run on either a BD LSRII or Symphony. A, B Significant increase in normalized iNKT (TCRβ+ CD1dtet+) cell numbers in distal duodenum. C Normalized cell numbers showing increased IL-10  +  expression in iNKT cells after both anastomotic surgeries. D Normalized iNKT cells that are IL-17A+ and IL-17F+ and then E iNKTh17 cells were gated for IL-22+ cells. *p  ≤  0.05–no surgery, n  =  5–8, except IL-10 and IL17A/F, n  =  8–10

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Gut Pathogens

ISSN: 1757-4749