Fig. 4

16 S rRNA gene sequencing results of gut microbiota of siblings of WT and Dock2^−/− mice. Feces were collected from WT and Dock2^−/− mice before and after cage division (4 weeks and 10 weeks). DNA was extracted, and submitted to16S rRNA gene sequencing analysis. A The heat map of the gene level based on the 30 most abundant OTUs in the sequencing results; B The microbiota abundance index Chao1 diversity analysis; C–F Anosim test results; G–K Metastats test results; L, M LEfSe test results; A represented the siblings of WT mice before cage division, B represented the siblings of Dock2^−/−mice before cage division, C represented siblings of WT mice at 6 weeks after cage division, and D represented the siblings of Dock2^−/− mice at 6 weeks after cage division. * p < 0.05, ** p < 0.01, ns, not significantly different