Table 1 Sequences of primers used in this study
Target | Primer (5ʹ to 3ʹ) | Amplicon sizes (bp) | TM (°C) |
|---|---|---|---|
LRRK2( DNA) (SNP Rs11564258) | F:ACCAAGGATACCCTAACTTCTTACCAA R: TCGATGGTGTTCTCAGCCCA | 330 | 62 |
LRRK2 (mRNA) | F:GGGTGCGAAGAGGACGAGGA R: AACCCACCTGCTGCACTC | 223 | 62 |
Dectin-1 | F:GATTTAGAAAATTTGGATGAAGATGG R:TATCACCAGTATTACCAAGCATA | 165 | 59 |
GAPDH | F: ATGCCTCCTGCACCACCAAC R: TGACCTTGCCCACAGCCTTG | 216 | 60 |