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Fig. 1 | Gut Pathogens

Fig. 1

From: Interaction between PSMD10 and GRP78 accelerates endoplasmic reticulum stress-mediated hepatic apoptosis induced by homocysteine

Fig. 1

Homocysteine promotes apoptosis of hepatocytes leading to liver injury. A The levels of Hcy in plasma and liver were measured by automatic biochemical analysis and ELISA in cbs+/+ and cbs+/− mice fed with high methionine diet (HMD) (n = 8/group). B The levels of serum aspartate aminotransferase (AST) and alanine transaminase (ALT) in cbs+/+ and cbs+/− mice were measured by automatic biochemical analysis. C The correlation between serum Hcy levels and AST or ALT levels were evaluated by Pearson correlation analysis. D Representative photomicrographs of hematoxylin & eosin (HE) staining in liver sections from cbs+/+ and cbs+/− mice (scale bars = 50 μm) E Representative immunofluorescence images of alive (green) and dead (red) hepatocytes after treatment with 100 μmol/L Hcy (scale bars = 100 μm). Nuclei were stained with DAPI (blue) F Apoptotic hepatocytes in the liver of cbs+/+ and cbs+/− mice were assessed by TUNEL staining (scale bars = 50 μm). G Representative western blot and quantification of Bax, Bcl-2, cleaved caspase-3 and cleaved caspase-12 in the liver tissue of cbs+/+ and cbs+/−mice. H The protein levels of Bax, Bcl-2, cleaved caspase-3 and cleaved caspase-12 were detected by western blot in hepatocytes treated with 100 μmol/L Hcy or Hcy + TUDCA for 48 h. I Apoptosis rate of hepatocytes was measured by flow cytometry after cells were treated with 100 μmol/L Hcy or Hcy + TUDCA for 48 h. The apoptotic indices are expressed as the number of apoptotic cells/the total number of counted cells × 100%. All data are expressed as mean ± SD. *P < 0.05, **P < 0.001 versus cbs+/+ mice or control group (without treated with Hcy); #P < 0.05, ##P < 0.01 versus Hcy group

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