Fig. 3

PSMD10 promotes hepatocytes apoptosis induced by homocysteine. A Co-immunofluorescent staining of PSMD10 (red) and CK18 (green, a positive marker for hepatocytes), nuclei were stained with DAPI (blue) (scale bars = 20 μm,). B The expression of PSMD10 was measured in the liver of cbs^+/−mice by western blot and qRT-PCR. C Western blot and qRT-PCR were employed to detect the expression of PSMD10 in the hepatocytes which was treated with 100 μmol/L Hcy. D, E The protein expression of GRP78, p-PERK, PERK, p-eIF2α, eIF2α, IRE1α, p-IRE1α, CHOP, cleaved caspase-3, cleaved caspase-12, Bax and Bcl-2 was analyzed by western blot, after the hepatocytes were transfected with PSMD10 siRNA (si-PSMD10) and treated with 100 μmol/L Hcy. F Apoptosis of hepatocytes was analyzed by flow cytometry after the cells were transfected with si-PSMD10 in the presence of Hcy. All data are expressed as mean ± SD. *P < 0.05, **P < 0.01 versus cbs^+/+, control or si-NC group. ^#P < 0.05, ^##P < 0.01 versus si-NC + homocysteine group