Fig. 1

Differential protein abundance following Rifaximin, Fidaxomicin and Myxopyronin B stress in C. difficile. The protein inventory of C. difficile 630 after stress with sublethal concentrations of Rifaximin (1.75 ng/ml; Rif), Fidaxomicin (6 ng/ml; Fid) and Myxopyronin B (500 ng/ml, MyxB) was analyzed by LC–MS/MS. A Proteins identified with at least two unique peptides in at least two out of three biological replicates but not in the DMSO controls or vice versa are displayed in a Venn diagram drawn with the R package “ggvenn”. B Bar chart presenting the percentage of differentially abundant proteins associated with displayed metabolic functions. C A data subset including only proteins that were significantly altered in their abundance according to DEqMS analysis or were absent in at least one condition was analyzed by hierarchical clustering of z-transformed intensity-based quantitative data using the R package “pheatmap”. More or unique abundance of proteins following stress compared to the DMSO controls is indicated by red coloring, lower abundance or absence following stress by black coloring. D Heatmap displaying proteins homogenously more abundant or only identified after treatment with all three antibiotics. E Heatmap displaying proteins specifically more abundant or only identified after treatment with Myxopyronin B. DMSO DMSO-treated samples, Rif Rifaximin-treated samples, Fid Fidaxomicin-treated samples, MyxB Myxopyronin B-treated samples, Bio1-3 biological replicates 1 to 3