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Fig. 1 | Gut Pathogens

Fig. 1

From: Curcumin assists anti-EV71 activity of IFN-α by inhibiting IFNAR1 reduction in SH-SY5Y cells

Fig. 1

EV71 blocks IFN-α signal in SH-SY5Y cells. SH-SY5Y cells were seeded in 6-plates and infected with neurotropic strain EV71 (Xiangyang-Hubei-09, GenBank Accession No. JN230523.1) at an MOI of 5 or treated with 2 μg/well Poly(dAT:dAT). 2 h and 12 h later, cellular supernatants were used to determined protein levels of supernatant IFN-α (a) and IFN-β (b) by ELISA following manufacturer’s instructions. c SH-SY5Y cells were firstly co-transfected with p-ISRE-FLuc (100 ng/well, 96-well plate) and p-RLuc (20 ng/well, 96-well plate) for 24 h, then the cells were infected with mock or EV71 for 10 h. After that, the cells were treated with 250 or 1000 IU/mL IFN-α for another 2 h. Intensity of FLuc and RLuc were measured as Materials and Methods described and Fluc/Rluc indicates ISRE activity. d SH-SY5Y cells seeded in 6-plates were treated in parallel as shown in c, total cellular protein were extracted and used for the detection of IFNAR1,IFNAR2, VP1 or β-actin protein by western blot, β-actin were used as loading control. All results indicate the mean ± SD of three independent experiments. Statistical analysis was performed using Student’s t-test, *P < 0.05, ** P < 0.01, *** P < 0.001, n.s. P > 0.05. EV, EV71; P(dAT), Poly(dAT:dAT); F, IFN-α

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