Fig. 3

Combination of Curcumin and IFN-α significantly inhibits EV71 replication. a SH-SY5Y cells were infected with EV71 at an MOI of 5. 2 h later, the cells were post-treated only with DMSO or IFN-α (250 IU/mL) or the combination of IFN-α with 5 or 20 μM Curcumin. At 12 h post infection, photomicrographs were taken (original magnification, 100×). b SH-SY5Y cells were infected with EV71 at an MOI of 5. 2 h later, the cells were post-treated only with DMSO or IFN-α (250 IU/mL) or the combination of IFN-α with 5 or 20 μM Curcumin. At 2,6,8,10,12 h post infection, intracellular progeny viruses were titrated using RD cells and the results presented as log₁₀ TCID₅₀/mL. c SH-SY5Y cells were co-transfected with p-ISRE-FLuc (100 ng/well, 96-well plate) and p-RLuc (20 ng/well, 96-well plate) for 24 h, then the cells were treated in parallel as shown in a, intensity of FLuc and RLuc was measured as Materials and Methods described and Fluc/Rluc indicates ISRE activity. d SH-SY5Y cells were treated with DMSO or IFN-α (250 IU/mL) or the combination of IFN-α with 5 or 20 μM Curcumin for 12 h followed by cell viability assessment using MTT. All the results indicate the mean ± SD of three independent experiments. Statistical analysis was performed using Student’s t-test, *P < 0.05, ** P < 0.01, *** P < 0.001, n.s. P > 0.05. EV, EV71; D, DMSO; F, IFN-α; C, Curcumin