Fig. 2

Experimental design of K. quasipneumoniae therapy and assessment of Klebsiella quasipneumoniae colonization. A The design of the study of mice gut colonization by K. quasipneumoniae and KvarIa treatment. All four experimental groups had antibiotic pretreatment, ampicillin therapy, bacterial administration, and different treatment for infection (PBS; uncoated-KvarIa; Eudragit S100-coated KvarIa 100 µg; Eudragit S100-coated KvarIa 1000 µg). Samples were collected on circled days. B Amplification of khe gene in mice faeces after the introduction of K. quasipneumoniae. khe gene was detected by RT-PCR. A lower CT value indicates a higher number of bacteria. The 4th day’s samples were used as a control group because they did not show any bacterial colonization. Statistically significant differences between mice groups (K. quasipneumoniae + Pen_ Strep + Amp vs K. quasipneumoniae + Pen_ Strep_Met + Amp) were not determined. Pen–penicillin, Strep–streptomycin, Met–metronidazole, Amp–ampicillin