Table 2 Predicted CTL epitopes from Enterococcus faecium penicillin binding protein to design multi-epitope vaccine construct with their corresponding MHC Class I alleles and their immunogenic properties
Uniprot_ID | CTL epitope | Alleles | Position | Score | Antigencity score | Immunogenicity | Toxicity | Hydrophobicity | Hydropathicity | Hydrophilicity | Charge | Mol wt |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
G5CKR9 | KLGDGGEKT | HLA-A*01:01, HLA-A*02:01, HLA-A*03:01, HLA-A*24:02, HLA-A*26:01, HLA-B*07:02, HLA-B*08:01, HLA-B*27:05, HLA-B*39:01, HLA-B*40:01, HLA-B*58:01, HLA-B*15:01 | 199 | 3.466 | 2.5192 | 0.05672 | Non-toxin | − 0.3 | − 1.43 | 1.09 | 0 | 904.12 |
KLIADKETK | HLA-A*03:01, HLA-A*26:01, HLA-B*07:02, HLA-B*08:01, HLA-B*27:05, HLA-B*39:01, HLA-B*40:01, HLA-A*01:01, HLA-A*02:01, HLA-A*24:02, HLA-B*58:01, HLA-B*15:01 | 567 | 0.395 | 0.4315 | 0.00835 | Non-toxin | − 0.37 | − 1.03 | 1.17 | 1 | 1045.37 | |
TTINWQPNL | HLA-A*24:02, HLA-B*07:02, HLA-A*01:01, HLA-A*02:01, HLA-B*08:01, HLA-A*03:01, HLA-A*26:01, HLA-B*27:05, HLA-B*39:01, HLA-B*40:01, HLA-B*58:01, HLA-B*15:01 | 145 | 3.248 | 1.4839 | 0.13021 | Non-toxin | − 0.09 | − 0.68 | − 0.8 | 0 | 1086.35 | |
EVDGRYYPL | HLA-B*58:01, HLA-A*01:01, HLA-A*02:01, HLA-A*03:01, HLA-A*24:02, HLA-A*26:01, HLA-B*07:02, HLA-B*08:01, HLA-B*27:05, HLA-B*39:01, HLA-B*40:01, HLA-B*15:01 | 259 | 0.794 | 1.6871 | 0.07862 | Non-toxin | − 0.21 | − 0.9 | 0.12 | − 1 | 1111.34 | |
LRGTTGGKL | HLA-B*27:05, HLA-A*24:02, HLA-A*26:01, HLA-B*07:02, HLA-B*58:01, HLA-A*01:01, HLA-A*02:01, HLA-A*03:01, HLA-B*08:01, HLA-B*39:01, HLA-B*40:01, HLA-B*15:01 | 311 | 2.321 | 1.9864 | 2.321 | Non-toxin | − 0.19 | − 0.38 | 0.18 | 2 | 902.2 |