Table 3 Predicted HTL epitopes from Enterococcus faecium penicillin binding protein to design multi-epitope vaccine construct with their corresponding MHC Class II alleles and their immunogenic properties
Uniprot_ID | MHC II epitope | Alleles | Pos | IC50 value | Percentile_Rank | Antigencity Score | Toxicity | Hydrophobicity | Hydropathicity | Hydrophilicity | Charge | Mol wt |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
G5CKR9 | DKFIFGEDLDLPISM | HLA-DRB1*01:01, HLA-DPA1*01:03, HLA-DPB1*02:01, HLA-DPB1*01:01, HLA-DRB1*09:01,HLA-DRB3*02:02, HLA-DRB1*13:02, HLA-DRB1*11:01, HLA-DRB1*04:01, HLA-DRB1*12:01, HLA-DPA1*03:01, HLA-DPB1*04:02, HLA-DRB1*04:05, HLA-DRB1*15:01, HLA-DQA1*01:01, HLA-DQB1*05:01, HLA-DRB1*08:02, HLA-DPA1*02:01, HLA-DPB1*14:01, HLA-DPB1*04:01, HLA-DQA1*05:01, HLA-DQB1*03:01, HLA-DQA1*04:01, HLA-DQB1*04:02, HLA-DPA1*02:01, HLA-DPA1*02:01, HLA-DPB1*05:01, HLA-DPA1*01:03, HLA-DQA1*05:01, HLA-DQB1*02:01, HLA-DQA1*03:01, HLA-DQB1*03:02, HLA-DQA1*01:02, HLA-DQB1*06:02, HLA-DRB3*01:01, HLA-DRB5*01:01, HLA-DRB1*07:01, HLA-DRB4*01:01, HLA-DRB1*03:01 | 502–516 | 19 | 0.87 | 0.8406 | Non-toxin | 0 | 0.23 | 0.12 | − 3 | 1740.22 |
DSLGGKAGSTVATTP | HLA-DRB5*01:01, HLA-DRB1*15:01, HLA-DRB3*01:01, HLA-DPA1*03:01, HLA-DPB1*04:02, HLA-DPA1*01:03, HLA-DPB1*02:01,HLA-DRB1*01:01, HLA-DRB1*09:01,HLA-DRB3*02:02, HLA-DRB1*13:02, HLA-DRB1*11:01, HLA-DRB1*04:01, HLA-DRB1*12:01,HLA-DRB1*04:05, HLA-DQA1*01:01, HLA-DQB1*05:01, HLA-DRB1*08:02, HLA-DPA1*02:01, HLA-DPB1*14:01, HLA-DPA1*01:03, HLA-DPB1*04:01, HLA-DQA1*05:01, HLA-DQB1*03:01, HLA-DQA1*04:01, HLA-DQB1*04:02, HLA-DPA1*02:01, HLA-DPB1*01:01, HLA-DPA1*02:01, HLA-DPB1*05:01,HLA-DQA1*05:01, HLA-DQB1*02:01, HLA-DQA1*03:01, HLA-DQB1*03:02, HLA-DQA1*01:02, HLA-DQB1*06:02, HLA-DRB1*07:01, HLA-DRB4*01:01, HLA-DRB1*03:01 | 357–371 | 12 | 1.3 | 1.9712 | Non-toxin | − 0.06 | − 0.15 | 0.07 | 0 | 1361.68 |