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Fig. 8 | Gut Pathogens

Fig. 8

From: Genome analysis and virulence gene expression profile of a multi drug resistant Salmonella enterica serovar Typhimurium ms202

Fig. 8

Expression profile of virulent genes in S. enterica Typhimurium ms202 under the influence of Mn2+. A Expression levels of nine different virulent genes encoding SPI1 (sit A, B, C, D, hilA, ompF and ssaV) and SPI2 (ssaB and sopB) regions of S. Typhimurium by qRT-PCR. Results are expressed as log2 fold change in S. enterica Typhimurium ms202 with respect to control LT2. 16 s rRNA gene was considered as a housekeeping gene in qRT-PCR. Error bars indicate Mean ± SE. The student's t-test determined statistical significance, and P < 0.05 was considered significant. B Expression levels of nine different virulent genes encoding SPI1 (sit A, B, C, D, hilA, ompF and ssaV) and SPI2 (ssaB and sopB) regions of S. Typhimurium by qRT-PCR after treatment of with 5uM MnCl2 compared to corresponding untreated controls. The 16 s rRNA gene was taken as the housekeeping gene in qRT-PCR. Error bars indicate Mean ± SE from three independent experiments. Statistical significance was determined by Student's t-test, and P < 0.05 was considered as significant

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