Fig. 1

A Sequence alignment of C. sakazakii phage-plasmid class members, pH322_1 and GK1025B_1: Annotated genomes of known phage-plasmids were aligned and compared to identify conserved and divergent sequence features. The annotation of each gene is from NCBI. The inner circle represents the sequence clockwise and the scale marks indicate positions of annotated genes. GenBank annotations of the reference pCsaC105731a (100,874 bp, coding DNA sequence (CDS) in black arranged outside ring), pCS1 (110,093 pb, Blue), pCsa767a (109,716 bp, purple), pCsaC757b (109,716 bp, Green), pGK1025B_1 (101,769 bp, Red) and pH322_1 (100,741 bp, Tan) was downloaded as GFF files for the analysis using the default configuration on the PROKSEE server. Across the circular genomes, selected genes or regions of interest are shown as follows: Missing regions identified by the BLAST analysis on the CGView server’s PROKSEE software are shown as ‘gaps’ on each of the circular genomes. The analysis was carried out on PROKSEE Server from the Stothard Research Group (University of Alberta, Canada) that uses BLAST analysis to illustrate conserved and missing genomic sequences (available online: https://beta.proksee.ca/tools). B Sequence Comparison of C. sakazakii virulence plasmid class members pH322_2 and pGK1025B_2: Annotated genomes of some known virulence plasmids were aligned and compared to identify conserved and divergent sequence features. The annotation of each gene is from NCBI. The inner circle represents the sequence clockwise and the scale marks indicate positions of annotated genes. GenBank annotations of the reference pESA3 (131,196 bp, CDS in black arranged outside ring), pSP291_1 (118,136 bp, Green), pH322_2 (118,185 bp, light blue), and pGK1025B_2 (120,182 bp, purple) was downloaded as GFF files for the analysis using the default configuration on the PROKSEE server. Across the circular genomes, selected genes or regions of interest are shown as follows: Franco et al. [31], adapted siderophore loci with Cronobactin gene, Iron ABC transporter genes, (T6SS), parAB genes, and the cpa gene. Missing regions identified by the BLAST analysis on the CGView server’s PROKSEE software are shown as ‘gaps’ on each of the circular genomes. The analysis was on the PROKSEE Server from the Stothard Research Group (University of Alberta, Canada) that uses BLAST analysis to illustrate conserved and missing genomic sequences (available online: https://beta.proksee.ca/tools)