Fig. 3

Sequence analysis of newly described C. sakazakii conjugative class member pGK1025B_3: Annotated genomes from pGK1025B_3 and other Cronobacter conjugative plasmids were compared using PROKSEE for identifying conserved and unique sequence features. The inner circle represents the sequence clockwise and the scale marks indicate positions of annotated genes. GenBank annotations of the reference pGK1025B_3 (46,528 bp, CDS in Black arranged outside ring), pC16KP0065-1 (168,415 bp, Teal), pC16KP0098-3 (49,279 bp, Purple), pC17KP0040-2 (56,619 bp, Dark Blue), pMG333 (134,435 bp, Tan), and pESA2 (31,208 bp, Green) were downloaded as a GFF file for analysis using the default configuration on the PROKSEE server. Across the circular genomes, selected genes or regions of interest are shown as follows: Missing regions identified by the BLAST analysis on the CGView server’s PROKSEE software are shown as ‘gaps’ on each of the circular genomes. The analysis was carried out on the PROKSEE Server from the Stothard Research Group (University of Alberta, CA) that uses BLAST analysis to illustrate conserved and missing genomic sequences (available online: https://beta.proksee.ca/tools)