Fig. 4

Fusobacterium nucleatum inhibited the antioxidant capacity of L02 cells. a ROS productions were detected by DHE staining. Representative images of the DHE staining in different groups. b ROS productions were evaluated by quantification of mean fluorescence intensity in DHE staining. c–e Levels of Malondialdehyde (MDA), Superoxide dismutase (SOD) and Glutathione peroxidase (GSH‐Px) in L02 cells. f Immunoblotting analysis on the protein levels of SIRT1 and quantified in (g). L02 cells stimulated with TNF-α (100 ng/mL) and D-Gal (44 μg/mL) were treated with F. nucleatum for 24 h. Data shown are means ± SD of three separate experiments. *P < 0.05; one-way ANOVA combined with Bonferroni's post hoc test; the error bars indicate the SDs