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Fig. 7 | Gut Pathogens

Fig. 7

From: Fusobacterium nucleatum promotes the development of acute liver failure by inhibiting the NAD+ salvage metabolic pathway

Fig. 7

Fusobacterium nucleatum inhibited NAD+ via the salvage synthesis pathway of NAD+. a Western blots analysis of p-AMPK in mice treated with LPS (100 μg/kg) and D-gal (400 mg/kg) or infected with F. nucleatum and quantified. b Western blots analysis of p-AMPK in L02 cells stimulated with TNF-α (100 ng/mL), D-Gal (44 μg/mL) and infected by F. nucleatum with a time gradient and quantified. c, d The cells were transfected with NAMPT plasmid or treated with AICAR for 24 h and then stimulated with TNF-α (100 ng/mL), D-Gal (44 μg/mL) and infected by F. nucleatum for 24 h. Western blotting was performed to measure the expression of SIRT1 and the NAD+ content of cells were measured in each group and quantified. e Representative images of the DHE staining in different groups and evaluated by quantification of mean fluorescence intensity (f). g immunofluorescence staining were performed to measure levels of pro-inflammatory cytokines and quantified in (h). Data shown are means ± SD of three separate experiments. *P < 0.05; one-way ANOVA combined with Bonferroni's post hoc test; the error bars indicate the SDs

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