Amoxicillin modulates gut microbiota to improve short-term high-fat diet induced pathophysiology in mice

Table 7 Effect of diet on mice gut microbiota at species level

Species	Relative abundance (%)				Significance (adjusted p value)
Species	CD	CD + A	HFD	HFD + A	CD vs CD + A	CD vs HFD	HFD vs HFD + A
Enterococcus casseliflavus	0.0 ± 0.000	0.0 ± 0.000	0.0 ± 0.000	2.6 ± 0.159	NS	NS	< 0.0001
Lactobacillus reuteri	0.2 ± 0.044	0.0 ± 0.000	0.0 ± 0.000	0.0 ± 0.000	< 0.0001	< 0.0001	NS
Blautia producta	0.1 ± 0.039	0.0 ± 0.000	0.1 ± 0.039	1.8 ± .0.132	< 0.0001	NS	< 0.0001
[Ruminococcus] gnavus	1.1 ± 0.104	0.1 ± 0.039	1.9 ± 0.136	0.1 ± 0.039	< 0.0001	< 0.0001	< 0.0001
Veillonella dispar	0.0 ± 0.000	0.0 ± 0.000	0.1 ± 0.039	0.4 ± 0.063	NS	0.0031	< 0.0001
Eubacterium dolichum	0.1 ± 0.039	0.0 ± 0.000	0.2 ± 0.044	16.6 ± 0.372	< 0.0001	NS	< 0.0001
Brevundimonas diminuta	0.0 ± 0.000	0.0 ± 0.000	0.1 ± 0.039	0.1 ± 0.039	NS	0.0002	NS
Haemophilus Parainfluenzae	0.0 ± 0.000	0.0 ± 0.000	0.3 ± 0.054	0.1 ± 0.039	NS	< 0.0001	0.0013
Bacteroides acidifaciens	0.1 ± 0.039	18.1 ± 0.385	0.1 ± 0.039	0.2 ± 0.044	< 0.0001	NS	0.0021
Bacteroides uniformis	0.0 ± 0.000	0.0 ± 0.000	0.0 ± 0.000	0.3 ± 0.054	NS	NS	< 0.0001
Prevotella melaninogenica	0.0 ± 0.000	0.0 ± 0.000	0.0 ± 0.000	0.3 ± 0.054	NS	NS	< 0.0001
Rothia mucilaginosa	0.0 ± 0.00	0.0 ± 0.00	0.7 ± 0.083	0.50 ± 0.070	NS	< 0.0001	0.0002
Akkermansia muciniphila	0.0 ± 0.00	0.1 ± 0.039	0.0 ± 0.00	0.10 ± 0.039	< 0.0001	NS	< 0.0001

Values are expressed as the mean ± SD (n = 6). Data were analyzed by One-way ANOVA followed by Bonferroni post-hoc test p ≤ 0.05, NS= not significant, CD: standard- chow diet for 3 weeks; CD + A: Standard- chow diet for 3 weeks and subjected to amoxicillin treatment (50 mg/kg body weight) in 3rd week; HFD: high-fat diet for 3 weeks (73% energy from fat); HFD + A: High-fat diet for 3 weeks and subjected to amoxicillin treatment (50 mg/kg body weight) in 3rd week

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