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Fig. 1 | Gut Pathogens

Fig. 1

From: Dietary and lifestyle associations with microbiome diversity

Fig. 1

Study Flow Diagram. Patients undergoing screening colonoscopy were screed for inclusion in the study. Of the 114 patients enrolled, 10 were excluded. Samples and data from the remaining 104 patients were used for data analysis. Stool samples were collected prior to bowel preparation using the Zymo Research DNA/RNA Shield Collection Tube and Tube. Samples were processed using the DNeasy PowerSoil Pro isolation kit. Sequencing was performed on an Illumina MiSeq instrument by amplification of the V4 region of the 16S rRNA. Questionnaires were obtained including patient medical history, dietary information and habits. Data was analyzed using R statistical software. To determine how other host metadata parameters influenced microbiome diversity, we performed a constrained ordination analysis. A stepwise model construction procedure for constrained ordination was then used to select a reduced model with P-value permutation

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