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Fig. 1 | Gut Pathogens

Fig. 1

From: Development and evaluation of a simple PCR assay and nested PCR for rapid detection of clarithromycin-resistant Helicobacter pylori from culture and directly from the biopsy samples in India

Fig. 1

Design of new MAMA PCR to identify clarithromycin resistant strain: Three separate primers, one common forward primer 2143F marked as brick-red for both sensitive and resistant type 23S rRNA and two allele-specific reverse primers, were designed with specific nucleotide at their respective 3ʹ ends; C for 2143 GR (complementary to resistant type allele marked in red) and T for 2143 AR (complementary to sensitive type allele, marked in green). Additionally, the 3ʹ mismatch effect was enhanced by alteration of another nitrogenous base in the second nucleotide position from the 3ʹ end of both the reverse primers (G in place of T being marked as violet). Parameter of the PCR is described schematically the condition of PCR

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Gut Pathogens

ISSN: 1757-4749