Skip to main content
Fig. 2 | Gut Pathogens

Fig. 2

From: CMTM3 protects the gastric epithelial cells from apoptosis and promotes IL-8 by stabilizing NEMO during Helicobacter pylori infection

Fig. 2

CMTM3 promotes IL-8 expression and inhibits the apoptosis of gastric epithelial cells. a CMTM3 expression in GES-1 and AGS cells were analyzed by western blotting. b The knockout efficiency of CMTM3 was analyzed by western blotting in GES-1 cells. c The overexpression efficiency of CMTM3 was analyzed by western blotting in AGS cells. d, e CMTM3 knockout (CMTM3 KO) and control (LentiV2) GES-1 cells were treated with H. pylori infection for 24 h. Levels of IL-8 were evaluated by qRT-PCR (d) and ELISA (e). f, g CMTM3 overexpression (pCMV-CMTM3) and control (pCMV) AGS cells were treated with H. pylori for 24 h. Levels of IL-8 were evaluated by qRT-PCR (f) and ELISA (g). h, i Representative Annexin V/propidium iodide staining of CMTM3 KO and control (LentiV2) GES-1 cells treated with H. pylori for 24 h (h) and the proportions of Annexin V-positive cells (i). *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page