Figure 4
From: Helicobacter pylori phagosome maturation in primary human macrophages
Co-localisation of LAMP-1 andLAMP-2 with H. pylori and E. coli phagosomes. Individual panels show the amount of co-localisation between the lysosome markers LAMP-1 (open square and dashed line) and LAMP-2 (closed square and solid line) and H. pylori (NL101, NL103, NL106, NL107, SS1) or E. coli (ECOL) phagosomes. Time-points were 0 minutes, 15 minutes, 30 minutes, two hours and four hours following initiation of phagocytosis. Co-localisation between the bacteria (FITC) and LAMP-1 and LAMP-2 markers (CY3) was quantified by calculating the overlap coefficients from confocal immunofluorescence images of macrophages containing approximately 100 phagosomes for each treatment, in duplicate.