Primer name and sequence | Purpose |
---|---|
LF:5′-AAAGGACATATTATCTATGCGTAATAAAATAATACTTGCCTGTGTAGGCTGGAGCTGCTT-3′ | Forward primer for amplification of the KnR gene cassette and upstream stdA flanking sequence |
LR:5′-CCGTGGACGGCTTCTCCCTGTCGTTATTTACCGCGTGAAACATATGAATATCCTCCTTAG-3′ | Reverse primer for amplification of the KnR gene cassette and downstream stdA flanking sequence |
F2:5′-CATCACCAACTCACCCTGTG-3′ | Forward primer for amplification of the stdA gene |
R2:5′-CTGAGGTATCTGCTGTGCCA-3′ | Reverse primer for amplification of the stdA gene |
F3:5′-ATTCATATGGTGCTTCGTTTAACACC-3′ | Forward primer for amplification of stdA for complementation |
R3:5′-AGACTCGAGTCACAGGTATTTCAGG-3′ | Reverse primer for amplification of stdA for complementation |
K3: 5′-AAAGCCACGTTGTGTCTA-3′ | Forward primer for amplification of the Knr gene cassette |
K5: 5′- CGCTGAGGTCTGCCTCGT-3′ | Reverse primer for amplification of the Knr gene cassette |