Global gene expression in Escherichia coli, isolated from the diseased ocular surface of the human eye with a potential to form biofilm

Table 1 Resistance profile of 12 ocular E. coli to nine antibiotics by the Kirby–Bauer disc diffusion method and their biofilm forming potential by and the tissue culture plate (TCP) method

S. no	E. coli strain	Ocular sample	Patient diagnosis	Resistance to antibiotics (µg/disc)	Biofilm production by TCP method ±
1	L-1339/2013	Conjunctival swab	Conjunctivitis	Ce, Cef, Ci, Ga, Ge, Mo, Of	−
2	L-1216/2010	Vitreous	Endophthalmitis	Ci	+
3	L-2561/2013	Vitreous	Endophthalmitis	None	+
4	L-1920/2011	Corneal scraping	Keratitis	Am, Ce, Cef, Ch, Ci, Ga, Ge, Mo, Of	+
5	L-3781/2010	Vitreous	Endophthalmitis	Ce	+
6	L-3484/2010	Vitreous	Endophthalmitis	None	−
7	L-1573/2013	Vitreous	Endophthalmitis	Ci, Mo, Of	+
8	L-494/2011	Vitreous	Endophthalmitis	Am, Ce, Cef, Ci, Ga, Ge, Mo, Of	+
9	L-223/2014	Corneal scraping	Keratitis	Ce, Cef, Ci, Ga, Mo, Of	+
10	L-304/2014	Corneal scraping	Keratitis	Ce, Cef	+
11	L-811/2014	Lacrimal sac	Dacryocystitis	Ce, Cef, Ci, Ga, Ge, Mo, Of	+
12	L-823/2014	Corneal scraping	Keratitis	Cef	+

The antibiotics used are (µg/disc): Am amikacin (30), Ce ceftazidime (30), Cef cefuroxime (30), Ch chloramphenicol (30), Ci ciprofloxacin (5), Ga gatifloxacin (5), Ge gentamicin (10), Mo moxifloxacin (5) and Of ofloxacin (5)
+, biofilm positive strains (black colonies); −, biofilm negative strains (red or pink colonies). biofilm positive strains (OD > 0.3 at 595 nm); −, biofilm negative strains (OD < 0.3) at 595 nm. The OD of the control wells was deducted in each case

ISSN: 1757-4749