Table 3 PCR and real-time PCR primers used in this study
Target gene | Primer sequence (5' -3') | Product size (bp) | Accession number | References |
|---|---|---|---|---|
wzx1-5d | F: CACTTGTTGGGTATGCTGG R: CCGGCAAACAGATTAGAAA | 782 | MN106906 | [4] |
gtrI | F: CTGTTAGGTGATGATGGCTTAG R: ATTGAACGCCTCCTTGCTATGC | 1122 | MN106907 | [4] |
gtrIc | F: AGGGAATGGCATTAGGGATCGG R: GCTGCAAGTGGTTTTTGTTGGA | 518 | MN106908 | [4] |
gtrII | F: ATTTATTGTTATTGGGGGTGGTTG R: ATTTGTTCTTTATTTGCTGGTT | 1272 | MN106909 | [4] |
gtrIV | F: ATGTTCCTCCTTCTTCCTTT R: TCCTGATGCTACCTTATCCA | 378 | MN099048 | [4] |
gtrV | F: AATACGATTCTCCTGGTGCTAAAC R: TAGGGCATTGCTTGTATCTTTCAT | 905 | – | [4] |
gtrX | F: AATGCTGGATGGGATAATCACCTT R: GAGACGGCTTCTCCATGTTTTGCT | 425 | MN106910 | [4] |
oac | F: CTGTTCGGCTTTGAAAGTGCTG R: CGTAGGCGTACATAGCAAGCAAAGA | 604 | MN106911 | [4] |
wzx6c | F: TTAAGAGCGATCATTTC R: CCATCCAAGCGGACATT | 739 | MN919548 | [4] |
16S rRNA | F: AACGTCAATGAGCAAAGGTATTAA R: TACGGGAGGCAGCAGTGG | 140 | – | [39] |
virB | F: GGAAGGGAGATTGATGGTAG R: GAACTTCAAGATCTGCTCCTGC | 84 | – | [39] |
ipaB | F: CTGCATTTTCAAACACAGC R: GAGTAACACTGGCAAGTC | 78 | – | [39] |
icsA | F: CTTTCGGGTACTCAAGAAC R: GAGAAAGTCCATCAACAGG | 76 | – | [39] |
icsB | F: CTCAATTCAACACTCTTTCACAG R: GCTGTACCGATGCCATGAAAAC | 82 | – | [39] (This study) |