Fig. 6

Histological evaluation of brain and intestinal tissue sections. A (X40, scale bar 50 µm) H&E staining of control intestinal tissue depicted normal intensity of goblet cells and lymphocytes in the lamina propria depicting normal tissue morphology. B (X40, scale bar 50 µm) Photomicrographs of Salmonella infected intestinal tissue depicted ileal damage inflicted by Salmonella with heavy influx of inflammatory cells along with crypt elongation (highlighted by arrows). C (X40, scale bar 50 µm) L. plantarum (RTA 8) treated group revealed restoration of normal intestinal tissue morphology as revealed by restoration of villi to crypt ratio and normal density of lymphocytes (highlighted by arrows). D (X40, scale bar 50 µm) Photomicrographs of cortical mice brain region in the control group depicted normal histology revealing presence of triangular neurons and glial cells. E (X40, scale bar 50 µm) Photomicrographs of brain tissue sections in the Salmonella infected group revealed heavy influx of inflammatory cells along the surface and meninges depicting meningitis (highlighted by arrows) along with brain infection characterized by oedema (highlighted by marking a circle). F (X40, scale bar 50 µm) Brain tissue sections of mice administered with L. plantarum (RTA-8) administered group depicted slight oedema with otherwise normal histoarchitecture