Table 2 Primers used for targeted gene expression analysis
Gene | Transcript ID | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) | Amplicon size, bp |
|---|---|---|---|---|
ACTB | NM_001101.5 | GGACTTCGAGCAAGAGATGG | TGTGTTGGCGTACAGGTCTTTG | 229 |
TLR4 | NM_138554.5 | ATATTGACAGGAAACCCCATCCA | AGAGAGATTGAGTAGGGGCATTT | 300 |
HSPA1A | NM_005345.6 | CCCCACCATTGAGGAGGTAG | ACATTGCAAACACAGGAAATTGA | 124 |
HSPB1 | NM_001540.5 | AAGCTAGCCACGCAGTCCAA | CGACTCGAAGGTGACTGGGA | 51 |
ZO1 | NM_003257.5 | CGGTCCTCTGAGCCTGTAAG | GGATCTACATGCGACGACAA | 371 |