Immunoblot cytokine array analysis of Caco-2 cells held at (A) 37°C, or exposed to (B) S . Typhimurium (ST) for 1 h at 37°C, (C) 41°C thermal stress for 1 h, or (D) 41°C thermal stress for 1 h followed by 1 h of S . Typhimurium exposure. (E) Template showing the position of different cytokines. Pos, positive control; Neg, negative control; B, blank; GCSF, granulocyte colony stimulating factor; GM-CSF, granulocyte monocyte-colony stimulating factor; I-309, a CC chemokine; TIMP-2, tissue inhibitor of metalloproteinase-2.