An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice

Table 2 The magnitude of GFP-specific CD8+ T cell responses as measured by IL-4 ELISPOT assay

Groups of mice were vaccinated three times (Days 0, 28 and 56) with live recombinant Salmonella vaccine expressing GFP (AroC+GFP) or a negative Salmonella control vaccine not expressing any antigen (AroC+pGEM). On Day 84 (28 days after the last inoculation), splenocytes from the sacrificed mice were incubated with media only (negative assay control), or stimulated with GFP CD8+ T cell peptide (HYLSTQSAL), full-length GFP or Salmonella LPS in an IL-4 ELISPOT assay. The mean number of spots ± SD in triplicate wells was calculated and expressed as IL-4 SFUs/10e6 cells. Difference in response between or within vaccines was determined. Responses differ significantly if the p-values are less than 0.05 and do not differ significantly if the p-values are greater than 0.05.

ISSN: 1757-4749