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Table 2 The magnitude of GFP-specific CD8+ T cell responses as measured by IL-4 ELISPOT assay

From: An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice

Stimulant IL-4 SFUs/10e6 cells p-value
  AroC+GFP Group AroC+pGEM Group  
Media 16 ± 10 12 ± 2 0.55
GFP CD8 peptide 148 ± 56 12 ± 13 0.015
GFP protein 18 ± 13 29 ± 26 0.56
Salmonella LPS 55 ± 29 26 ± 9 0.17
Vaccine group Media GFP peptide p-value
AroC+GFP 16 ± 10 148 ± 56 0.016
AroC+pGEM 12 ± 2 12 ± 13 0.97
  1. Groups of mice were vaccinated three times (Days 0, 28 and 56) with live recombinant Salmonella vaccine expressing GFP (AroC+GFP) or a negative Salmonella control vaccine not expressing any antigen (AroC+pGEM). On Day 84 (28 days after the last inoculation), splenocytes from the sacrificed mice were incubated with media only (negative assay control), or stimulated with GFP CD8+ T cell peptide (HYLSTQSAL), full-length GFP or Salmonella LPS in an IL-4 ELISPOT assay. The mean number of spots ± SD in triplicate wells was calculated and expressed as IL-4 SFUs/10e6 cells. Difference in response between or within vaccines was determined. Responses differ significantly if the p-values are less than 0.05 and do not differ significantly if the p-values are greater than 0.05.