Isolation and detection of Mycobacterium avium subsp. paratuberculosis (MAP) from cattle in Ireland using both traditional culture and molecular based methods

Table 2 PCR and Culture Results

			No. of samples positive (percentage)
Herd Id	Sampling	No. of samples	Culture		PCR
			MAP	Other	IS900	ISMAP02 (1)	ISMAP02 (2)
H1	11/2006	4	3 (75)	0	4 (100)	4 (100)	4 (100)
	05/2007	22	7 (29.7)	0	22 (100)	22 (100)	22 (100)
H2	05/2007	44	6 (13.6)	0	44 (100)	44 (100)	44 (100)
	04/2008	73	0	18 (24.6)	0	0	6 (8.2)
H3	07/2007	35	5 (14.3)	0	14 (40)	14 (40)	20 (57.1)
H4	07/2007	62	2 (3.2)	0	21 (33.9)	21 (33.9)	34 (54.8)
H5	04/2008	17	0	3 (17.6)	0	0	1 (5.9)
H6	04/2008	2	0	0	0	0	0
H7	10/2008	31	0	1 (3.2)	0	0	3 (9.6)
Total		290	23 (7.9)	22 (7.5)	105 (36.2)	105 (36.2)	134 (46.2)

Data illustrating the results for each herd grouped according to PCR (IS900 & ISMAP02), nested PCR (ISMAP02), culture and non-MAP culture. In total seven herds were sampled over the 2 year period and the number of animals sampled ranged from 2 - 73. When examined collectively on a herd basis a 100% correlation was shown to exist between PCR and culture, ie the overall first round PCR results could be used to predict whether any animal in that herd would show up later as being culture positive (H1,2 ['07],3,4) or negative (H2['08], H5, H6, H7).

ISSN: 1757-4749