Skip to main content

Table 2 PCR and Culture Results

From: Isolation and detection of Mycobacterium avium subsp. paratuberculosis (MAP) from cattle in Ireland using both traditional culture and molecular based methods

    No. of samples positive (percentage)
Herd Id Sampling No. of samples Culture PCR
    MAP Other IS900 ISMAP02 (1) ISMAP02 (2)
H1 11/2006 4 3 (75) 0 4 (100) 4 (100) 4 (100)
  05/2007 22 7 (29.7) 0 22 (100) 22 (100) 22 (100)
H2 05/2007 44 6 (13.6) 0 44 (100) 44 (100) 44 (100)
  04/2008 73 0 18 (24.6) 0 0 6 (8.2)
H3 07/2007 35 5 (14.3) 0 14 (40) 14 (40) 20 (57.1)
H4 07/2007 62 2 (3.2) 0 21 (33.9) 21 (33.9) 34 (54.8)
H5 04/2008 17 0 3 (17.6) 0 0 1 (5.9)
H6 04/2008 2 0 0 0 0 0
H7 10/2008 31 0 1 (3.2) 0 0 3 (9.6)
Total 290 23 (7.9) 22 (7.5) 105 (36.2) 105 (36.2) 134 (46.2)
  1. Data illustrating the results for each herd grouped according to PCR (IS900 & ISMAP02), nested PCR (ISMAP02), culture and non-MAP culture. In total seven herds were sampled over the 2 year period and the number of animals sampled ranged from 2 - 73. When examined collectively on a herd basis a 100% correlation was shown to exist between PCR and culture, ie the overall first round PCR results could be used to predict whether any animal in that herd would show up later as being culture positive (H1,2 ['07],3,4) or negative (H2['08], H5, H6, H7).