Figure 3
From: Helicobacter pylori phagosome maturation in primary human macrophages
Co-localisation of late endosome markers with H. pylori and E. coli phagosomes. Individual panels show the amount of co-localisation between the late endosome markers Rab7 (open triangle and dashed line) and CD63 (closed triangle and solid line) and H. pylori (NL101, NL103, NL106, NL107, SS1) or E. coli (ECOL) phagosomes. Time-points were 0 minutes, 15 minutes, 30 minutes, two hours and four hours following initiation of phagocytosis. Co-localisation between the bacteria (FITC) and late endosome markers (CY3) was quantified by calculating the overlap coefficients from confocal immunofluorescence images of macrophages containing approximately 100 phagosomes for each treatment, in duplicate.