H. pylori megasomes. Confocal immunofluorescence micrographs of megasomes in primary human macrophages 30 minutes after infection with H. pylori strain NL101 (A) or NL107 (B). Infected macrophage are immune stained with rabbit anti-H. pylori serum/donkey anti-rabbit IgG FITC conjugate (green), mouse anti-CD63/donkey anti-mouse IgG CY3 conjugate or sheep anti-LAMP-1/donkey anti-sheep IgG CY3 conjugate (red) and nuclear DNA stained with DAPI (blue). Arrows designate megasomes containing multiple intact H. pylori in overlay and single channels. Scale bar = 5 μm.