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Table 1 Genetic loci identified by IS3 profiling

From: IS3 profiling identifies the enterohaemorrhagic Escherichia coli O-island 62 in a distinct enteroaggregative E. coli lineage

Band ref no. Band size Criteria for selection Sequence showing > 90% similarity at the nucleotide level (Genbank Accession #) Subsequent evaluation of locus
650 1.7 kb Present in several (13/22) EAEC, absent in K12 E. coli 2-acylglycerophosphoethanolamine acyltransferase/acyl-acyl carrier protein synthetase gene (gi|290402) Gene is present in the K-12 genome
651 1.2 kb Present in ST31 and ST394 EAEC strains. Absent in all others. orfz2240 (unknown function) from "O-island #62" of E. coli O157:H7 EDL933 genome (gi|12515207|gb|AE005358.1) Present in ST31, ST38 and ST394-complex EAEC strains and EHEC O157. absent in other EAEC
652 0.35 kb Present in K12 and EDL933, absent in Shigella and 16/22 EAEC. No phylogenetic association. E. coli racC and recE genes, complete cds and 5' end (gi|147534|gb|M24905.1|ECORECEA) Present in EAEC
708 0.65 kb Absent in ST10 EAEC, present in most other strains E. coli 23 S rRNA gene, strain K12 DSM 30083T (gi|12053855|emb|AJ278710.1) 23 S rRNA, no diagnostic potential
711 0.5 kb Present in 8/22 EAEC strains, and Shigella, absent in other strains E. coli aggR gene for fimbrial adhesin activator (gi|471301|emb|Z32523.1|ECAGGRG) Previously described plasmid-borne EAEC gene [17]
712 1.3 Kb Absent in most EAEC (19/22), Present in K12 E. coli glycine decarboxylase (gi|NC 000913|U00096) Gene is present in EAEC strains