Figure 1

Study overview. The key events associated with the Clostridium difficile induced colitis mouse model are shown. On study days 0 through 3, C57BL/6 mice received an antibiotic mixture of kanamycin, gentamicin, colistin, metronidazole and Vanco in the drinking water, followed by clindamycin (10 mg/kg, i.p., on day 5). On day 6, the C. difficile strain VPI 10463 was given by oro-gastric gavage at ≈ 5x10⁴ CFU to induce colitis. Mice were treated on study days 6 to 10 with Vanco (50 mg/kg) or vehicle (saline) by gavage. On days 10 to 16, mice were dosed by gavage with vehicle (50% maltodextrin/saline, n=29) or BC30 (2 x 10⁹ CFU per day, n=28). One negative control group of mice (n=6) was dosed with vehicle, but did not receive C. difficile, while a positive control group (initial n of 8) received C. difficile but not Vanco. Mice were monitored daily (days 6 to 17) for mortality, weight loss and stool consistency. On study days 14, 16 and 17, stools and colons were collected for further analyses.