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Figure 5 | Gut Pathogens

Figure 5

From: Secreted Listeria adhesion protein (Lap) influences Lap-mediated Listeria monocytogenes paracellular translocation through epithelial barrier

Figure 5

Transepithelial translocation characteristics of L. monocytogenes. (a and b) Transepithelial translocation characteristics of L. monocytogenes through paracellular route in Caco-2 cell monolayers by trans-well assays. (c) DextranFITC permeability through Caco-2 cell monolayer in trans-well model and (d) Transepithelial electrical resistance (TEER) in Caco-2 cell monolayers after treatment with high (H4, H8, H13) and low (CHLR4, CHLR6, ATCC 19117) Lap secreting isolates. L. monocytogenes F4244 (WT), lap mutant (lap-), lap complemented (lap+), ΔinlA and inlA complemented (inlA+) strains were used as controls. Data are average of at least three independent experiments performed in triplicate (n ≥ 9). Bars marked with different letters (a,b,c) are significantly different at P < 0.05.

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