LGG stimulates apoptosis in Gn pig ileum treated with HRV. (A) LGG increases the expression of p53 protein and decreases Bcl-2 and Bcl-xl protein in the ilea of HRV-infected pigs. Ileum epithelia were scraped on PID 2. Relative protein band intensities of P53 in pig ileum were analyzed with NIH image. Data are reported as the mean ± SD. ANOVA * P<0.05. (B) LGG increases the expression of cleaved caspase-3 in the ilea of HRV-infected pigs. Densitometry analysis of caspase-3 activation. Values are the ratio of cleaved caspase-3 to pro-caspase-3. The results were normalized to mock (densitometer value = 1). * P<0.05. (C) Detection of p53 and cleaved caspase-3 by immunofluorescence in pig ileum (Magnification 20×, scale bar 100 μm).