Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines

Table 4 Primers for VAGs of E. coli

Genes		Primer and probe sequences	Fragment length	Accession	Source of primer
		(5′ 3 f: forward; r: reverse)
Multiplex PCR
ipa H	f	GTTCCTTGACCGCCTTTCCGATACCGTC	619	M32063	[17]
ipa H	r	GCCGGTCAGCCACCCTCTGAGAGTAC	619	M32063	[17]
daa D	f	TGAACGGGAGTATAAGGAAGATG	371	AY525531.1	[17]
daa D	r	GTCCGCCATCACATCAAAA	371	AY525531.1	[17]
elt B	f	TCTCTATGTGCATACGGAGC	322	EU113252.1	[17]
elt B	r	CCATACTGATTGCCGCAAT	322	EU113252.1	[17]
eae A	f	CCTGGTTACAACATTATGGAACG	287	AJ308550.1	[4]
eae A	r	TGAAATAGTCTCGCCAGTATTCG	287	AJ308550.1	[4]
stx 2	f	GGCACTGTCTGAAACTGCTCC	255	FN252459	[17]
stx 2	r	TCGCCAGTTATCTGACATTCTG	255	FN252459	[17]
agg R	f	CGTAAGCCGGGTATGAAAGA	188	Z32523.1	[4]
agg R	r	GCCAGTTCAGAAGCAGGAAC	188	Z32523.1	[4]
est 1a	f	TTTCCCCTCTTTTAGTCAGTCAA	159	M25607.1 AY342057.1	[17]
est 1a	r	GCAGGATTACAACACAATTCACAGCAG	159	M25607.1 AY342057.1	[17]
stx 1	f	CTGGATTTAATGTCGCATAGTG	150	HM367099.1	[17]
stx 1	r	AGAACGCCCACTGAGATCATC	150	HM367099.1	[17]
est 2	f	CTATTGCTACAAATGCCTATGC	126	M35586.1	[4]
est 2	r	CTCCAGCAGTACCATCTCTA	126	M35586.1	[18]
Single PCR
pEAF	f	CAGGGTAAAAGAAAGATGATAA	397	X76137.1	[16]
	r	TATGGGGACCATGTATTATCA

ISSN: 1757-4749