Table 1 Bacterial strains and plasmids
From: Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance
Strain or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
Plasmids | ||
pUC18 | Ampr, lacZ', pMB9 replicon | [13] |
pUC18::ESA_02131 | pUC18 with ESA_02131 gene under control of native promoter | This work |
pUC18::ESA_01706 | pUC18 with ESA_01706 gene under control of native promoter | This work |
pUC18::ESA_04214 | pUC18 with ESA_04214 gene under control of native promoter | This work |
pUC18::ESA_pESA3p05450 | pUC18 with ESA_pESA3p05450 gene under control of native promoter | This work |
pUC18::ESA_01226 | pUC18 with ESA_01226 gene under control of native promoter | This work |
pUC18::ESA_00673 | pUC18 with ESA_00673 gene under control of native promoter | This work |
pUC18::ESA_03328 | pUC18 with ESA_03328 gene under control of native promoter | This work |
Strains | ||
Cronobacter sakazakii BAA-894 | C.sakazakii strain isolated from powdered formula associated with neonatal intensive care unit | [12] |
Escherichia coli DH5α | Intermediate cloning host.supE44 ΔlacU169(80lacZΔM15)R17 recA1 endA1 gyrA96 thi-1 relA1. | Invitrogen. |
MKH13 | MC4100Δ(putPA)101Δ(proP)2Δ(proU) | [15] |
MKH13 pUC18::ESA_02131+ | Host strain harbouring pUC18: ESA_02131 plasmid. Ampr. | This work |
MKH13 pUC18::ESA_01706+ | Host strain harbouring pUC18: ESA_01706 plasmid. Ampr. | This work |
MKH13 pUC18::ESA_04214+ | Host strain harbouring pUC18: ESA_04214 plasmid. Ampr. | This work |
MKH13 pUC18::ESA_pESA3p05450+ | Host strain harbouring pUC18: ESA_pESA3p05450 plasmid. Ampr. | This work |
MKH13 pUC18::ESA_01226+ | Host strain harbouring pUC18: ESA_01226 plasmid. Ampr. | This work |
MKH13 pUC18::ESA_00673+ | Host strain harbouring pUC18: ESA_00673 plasmid. Ampr. | This work |
MKH13 pUC18::ESA_03328+ | Host strain harbouring pUC18: ESA_03328 plasmid. Ampr. | This work |