Systemic and extra-intestinal pro-inflammatory cytokine responses following ileitis induction. Serum (A) IL-6 and (B) IFN-γ, splenic (C) TNF-α and (D) IFN-γ as well as hepatic (E) TNF-α and (F) IL-6 protein concentrations were determined in respective ex vivo biopsies derived from C57BL/6 wildtype (WT; black circles) and TLR-9 deficient (TLR-9-/-; white circles) mice seven days following ileitis induction (ILE). Naïve mice served as negative controls (N). Numbers of analyzed mice (in parentheses), means (black bars) and levels of significance (P-values) as compared to the respective groups (determined by Mann–Whitney-U test) are indicated. Data shown were pooled from three independent experiments.