Figure 2

Cell line specific responses and gene signatures of active and persistent C. trachomatis infection. A. Differentially transcribed genes (up or down-regulated by >2-fold) in active (AI) or persistent (PI) infection of LCC-18 and CNDT-2 cells compared to non-infected cells (NI). We identified 66 differentially transcribed genes (61 up-regulated) in LCC-18 cells with active C. trachomatis infection compared to non-infected cells and 411 (108 up-regulated) in persistently infected LCC-18 cells compared to non-infected cells. Twelve differentially transcribed genes (11 up-regulated) were identical for both active and persistent infection of LCC-18 cells. In CNDT-2 cells, we identified 68 differentially transcribed genes when infected with C. trachomatis (58 up-regulated) and respectively 170 differentially transcribed genes or ESTs (120 up-regulated) in persistently infected CNDT-2 cells compared to non infected cells. Thirty-nine differentially transcribed genes (all up-regulated) were identical for both active and persistent infection of CNDT-2 cells. Baculoviral IAP repeat-containing 3 (BIRC3) was the only gene that was up-regulated in both cell lines during active and persistent infection. B. Schematic presentation of differentially transcribed genes clusters in active (AI) and persistent (PI) infection compared to non-infected cells (NI) in LCC-18 and CNDT-2 cells. Gene clustering based on Gene Ontology (GO) was performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) (http://david.abcc.ncifcrf.gov).