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Figure 4 | Gut Pathogens

Figure 4

From: Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serum

Figure 4

C3 complement protein depositions on immobilized LPS. Direct Sandwich ELISA. Microtiter plate wells were coated for 2 h at 37°C with polyclonal rabbit anti-C3c diluted 1/500 in 0.1 M sodium carbonate buffer (pH 9.6). Mixtures of LPS (0.6; 6.0; 60.0 μg/ml) and 80% HS were incubated for 45 min at 37°C. Activation of C3 differs significantly if the p values are less than p < 0.05 (*).

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