Figure 4From: Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serumC3 complement protein depositions on immobilized LPS. Direct Sandwich ELISA. Microtiter plate wells were coated for 2 h at 37°C with polyclonal rabbit anti-C3c diluted 1/500 in 0.1 M sodium carbonate buffer (pH 9.6). Mixtures of LPS (0.6; 6.0; 60.0 μg/ml) and 80% HS were incubated for 45 min at 37°C. Activation of C3 differs significantly if the p values are less than p < 0.05 (*).Back to article page