Figure 5From: Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serumImmunoblot detection of C3c fragments on native OMP. OMP isolated with Zwittergent Z 3–14 detergent®. OMP patterns were determined by blue native polyacrylamide gel electrophoresis (BN-PAGE) (a) and C3 binding confirmed by Western blotting (b). Electrotransfer conducted at 100 V for 1 h. Lane 1 molecular-weight marker 26625 (Thermo Scientific). The OMP concentrations were 10 μg/well (*), and 12 μg/well (**), respectively.Back to article page