Immunoblot detection of C3c fragments on native OMP. OMP isolated with Zwittergent Z 3–14 detergent®. OMP patterns were determined by blue native polyacrylamide gel electrophoresis (BN-PAGE) (a) and C3 binding confirmed by Western blotting (b). Electrotransfer conducted at 100 V for 1 h. Lane 1 molecular-weight marker 26625 (Thermo Scientific). The OMP concentrations were 10 μg/well (*), and 12 μg/well (**), respectively.