Figure 6From: Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serumImmunoblot detection of C3c fragments on outer membrane proteins (OMP) under reducing conditions. OMP isolated with Zwittergent Z 3–14 detergent® OMP patterns were determined by sodium dodecyl suphate-polyacrylamide gel electrophoresis (SDS–PAGE) (10 μg/well) (a) and C3 binding confirmed by Western blotting, (20 μg/well) (b). Electrotransfer conducted at 50 V for 1 h. Lane 1 molecular-weight marker A8889 (AppliChem).Back to article page