Fig. 3
Kinetics of immune cell infiltration in ileal tissue following murine A. butzleri infection. Gnotobiotic IL-10−/− mice were generated by antibiotic treatment and orally infected either with A. butzleri strain CCUG 30485 (circles) or strain C1 (squares). Uninfected gnotobiotic IL-10−/− mice served as negative control (black diamonds). The average number of cells positive for a CD3 (T lymphocytes). b FOXP3 (regulatory T cells, Tregs). c B220 (B lymphocytes) and d F4/80 (macrophages and monocytes) from at least six high power fields (HPF, ×400 magnification) per animal were determined microscopically in immunohistochemically stained ileal sections derived from mice at day 6 p.i. (filled symbols) and day 16 p.i. (open symbols). Numbers of analyzed animals are given in parentheses. Medians (black bars) and significance levels as determined by the Mann–Whitney U test are indicated. Data shown were pooled from three independent experiments