Table 2 List of primers in this study
Primer | Sequence | Purpose |
|---|---|---|
PUT-F3A | 5′-CAGGAGTACGGATAAAATGC-3′ | Confirmation of genotype after insertion mutagenesis |
PUT-R1 | 5′-AAGGTTTAACGGTTGTGGAC-3′ | |
K2-wzy-IF | 5′-ATTTTCCAGAGTTAGACCCG-3′ | Construction of TSGH69Δwzy |
K2-wzy-IR | 5′-TGTCGTTTTGGGATTTGTAA-3′ | |
K2-wzy-OF | 5′-GCCTTTTCATTTATACAGGA-3′ | Confirmation of TSGH69Δwzy genotype |
K2-wzy-OR | 5′-GGAATTGAAATCAACTACAG-3′ | |
K2-wbbO-IF | 5′-GGAATTCCTGTTTGATTGGTGGTGTGCT-3′ | Construction of TSGH69ΔwbbO |
K2-wbbO-IR | 5′ATAAGAATGCGGCCGCGACGGCAAAGCAACGATATT-3′ | |
K2-wbbO-OF | 5′-AGATTCACATCAGCCATTTT-3′ | Confirmation of TSGH69ΔwbbO genotype |
K2-wbbO-OR | 5′-GGAATTCCTGAGAAAATTGTGTTATTTCA-3′ |