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Fig. 6 | Gut Pathogens

Fig. 6

From: De Novo sphingolipid synthesis is essential for Salmonella-induced autophagy and human beta-defensin 2 expression in intestinal epithelial cells

Fig. 6

The involvement of PI3 K/Akt on Salmonella-induced IL-8 and hBD-2 mRNA expression. SW480 cells were left untreated, or treated with 50 μM LY294002 (LY). They were then infected with the wild-type S. Typhimurium strain SL1344 for 1 h. Total RNA was prepared and analyzed by real-time quantitative PCR to estimate amounts of IL-8 and hBD-2 transcript. The amount of IL-8 and hBD-2 mRNA expression, normalized to the corresponding amount of GAPDH transcript, is shown as the fold increase over uninfected, control cells (CON). Results are represented as mean ± S.E.M. for at least three determinations from independent experiments. An asterisk indicates a significant difference (p < 0.05)

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Gut Pathogens

ISSN: 1757-4749