Fig. 1
The effect of C. concisus Zot on TEER. Caco-2 cells grown for 21 days were treated apically with two concentrations (a 12.5 μg/100 μl, b 50 μg/100 μl) of EP and EP-ZotP14UCO-S1 proteins dissolved in DPBS. Caco-2 cells in DPBS were utilized as the negative control. Following 4 h of incubation, the proteins and DPBS were removed and cells were further incubated. TEER was measured at different time points. TEER was expressed as the percentage relative to TEER reading at time 0. Dotted lines indicate lapsed times between 8 and 24 h. The levels of TEER in Caco-2 cells treated with EP-ZotP14UCO-S1 and EP were compared. The TEER values for DMSO treated cells were 59 ± 4.0 %. * Indicates P < 0.05 and *** Indicates P < 0.001. Data are shown as the mean ± SD from triplicates and are representative of three independent experiments. EP-ZotP14UCO-S1: constituted of E. coli proteins and the Zot of C. concisus strain P14UCO-S1 expressed in E. coli. EP control E. coli proteins. TEER transepithelial electrical resistance