Fig. 2From: Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samplesBackground levels of bacterial genomes based on 16S rRNA gene universal primers suggest a level of at least 4 bacterial genomes per µl of qPCR reaction mixture. Although the true amount of contaminating bacterial DNA would be 4 + NTC, assuming the lowest possible figure, a standard 50 µl qPCR reaction would contain at least 200 E. coli-equivalent genomes or approximately 1400 rRNA gene copies contaminating the reactionBack to article page